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Transgenic banana

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1) Over 600 banana plants were produced and screened for BBTV resistance using aphid bioassay 2) More than 60 plants showed initial resistance to BBTV (delay in the expression of symptoms compared to non-transgenic banana plants) 3) 3 plants remained symptom-free after two challenges, but eventually became symptomati Banana is probably the most important fruit crop in the world with an annual production of more than 80 million tons (Anonymous 1995). In many of the 120, mostly less-developed banana producing countries, banana is locally consumed fresh, prepared by cooking or processed for chips and other food products. In fact, it is (after rice, milk and. An Agrobacterium -mediated plant transformation system was developed for the generation of transgenic banana (Musa spp. van Grand Nain). This system allowed for the recovery of putative..

Transgenic banana plants expressing the Sm-AMP-D1 gene were subsequently generated via Agrobacterium-mediated genetic transformation. Transgenic nature of the regenerated banana plants was confirmed by genomic DNA PCR and Southern blotting analysis. Northern blots demonstrated efficient expression of Sm-AMP-D1 mRNA in transgenic banana plants Transgenic banana cv. Cavendish Williams and plantain cvs. Gonja Manjaya and Orishele expressing AChE dsRNA were regenerated and assessed for transgene integration and copy number. When aphids were maintained on elite transgenic events, there was a 67.8%, 46.7%, and 75.6% reduction in aphid populations growing on Cavendish Williams, Gonja. Transgenic approach has also been used to incorporate the desirable traits into banana. Recent advances in genomics and the availability of genome sequence of both Musa acuminata and Musa balbisiana helps in the improvement of this fruit crop transgenic banana plants, along with the non-transgenic control group. To increase the exposure to the fungus, we buried TR4-infected banana plant material between each plant and reinfested the soil heavily. we regularly inspected plants for TR4 symptoms, such as wilting and leaf yellowing

The authors report on their 3-year field trial of two transgenic lines of Cavendish banana, one that is transformed with RGA2, a gene that is found in south Asian diploid banana subspecies. RGA 2 homologs are naturally present in Cavendish but are expressed at a low level Previously, transgenic resistance to Foc race 1 in banana has been reported from glasshouse testing using either anti-apoptosis genes 8, 15 or RNA interference targeting essential Foc genes 16... Banana: Genomics and Transgenic Approaches for Genetic Improvement. Provides all the up to date literature on banana genomics. Covers state -of- the- art technologies on banana genetic manipulation. Provides complete information on the transgenic banana plants developed and field tested. see more benefits

The development of Xcm-resistant banana using the transgenic approach is a significant technological advance that will increase the available arsenal of weapons to fight the BXW epidemic and save livelihoods in Africa. It can become a high-value product for farmers. This research is supported by the Gatsby Charitable Foundation, AATF, and USAID Transgenic banana plants were obtained for both pMSI164 and pMSI168 transformations and displayed considerable resistance to F. oxysporum f. sp. cubense and Mycosphaerella musicola. The obtained results suggest that MSI-99 can be successfully applied in imparting enhanced disease resistance in transgenic banana plants (Chakrabarti et al., 2003) Transgenic bananas have been generated by inserting Hrap or Pflp gene in embryogenic cell suspensions of banana cultivars, the AAB sweet banana cultivar 'Sukali Ndiizi', and the AAA-EAHB cultivar 'Nakinyika', through Agrobacterium- mediated transformation (Tripathi et al. 2010; Namukwaya et al. 2012) Transgenic and wild-type banana lines established in tissue culture were transported to the field trial site at the Department of Agriculture and Fisheries (DAF) South Johnstone Research Facility (Queensland, Australia) according to conditions on the Office of the Gene Technology Regulator (OGTR) licence number DIR109 BBTV-resistant transgenic banana is used as an example to demonstrate our specific approaches. Banana is one of the most widely planted fruits in the world and is a staple for millions of people worldwide. We have produced transgenic Dwarf Brazilian banana that are resistant to BBTV, the most serious viral disease of banana

Transgenic Banana ( Musa Species) SpringerLin

The transgenic banana carrying these genes may also display resistance to fungal diseases such as black sigatoka and Fusarium wilt. Experiments on this are being conducted in our lab in Uganda. Confined field trial of banana plants. Source: L. Tripathi, IITA Transgenic approaches are potential tools for direct transfer of these genes into popular cultivars, which are generally not amenable for conventional breeding techniques, in specific with crops such as bananas which are sterile, triploid and heterozygous thereby making it difficult to reconstruct the recurrent genotypes in banana. Transgenic. He does this by inserting genetic material from other organisms into banana plants. This work is separate from the research that our foundation supports, although some of the same techniques and scientific principles are involved, including transgenic experiments. Making banana plants less susceptible to diseases is a secondary goal for us Transgenic banana plants Gongjiao (Musa acuminata L. AA group, cv. Mas) were generated using the Agrobacterium mediated method with a high transformation efficiency as described in our previous study (Liu et al., 2017). The hygromycin-resistant transgenic lines were validated by PCR amplification of the hpt-Ⅱ gene in the binary vector

Generation of Transgenic Banana ( Musa acuminata ) Plants

The transgenic banana plantlets were inoculated with 2x10 6 spores mL-1 conidial suspension of Fusarium oxysporum f. sp. cubense (Race 1) to evaluate the degree of tolerance and to investigate the effectiveness of the bioassay system as a potential tool for early screening. Assay of protein extract from the transgenic plantlets showed an. individual transgenic plants harboring the HL gene were obtained and 24 displayed resistance to FocR4 in the early vegetative growth stage. Hence, Agrobacterium-mediated transformation with the assistance of particle bombardment is a way in which a larger number of transgenic banana plants may be obtained Biotechnology and transgenic technology, together with conventional methods can assist in overcoming these problems in developing new banana cultivars. Some successes in genetic engineering of Musa have been achieved, enabling the transfer of foreign genes into the plant cells. The transgenic approach shows potential for the geneti Production of Transgenic Banana (Musa species) Transgenic Plants and Crops, 2002. Laszlo Sagi. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper. READ PAPER. Production of Transgenic Banana (Musa species) Download

This is a significant step toward development of transgenic banana cultivars resistant to BXW, which will boost the available arsenal to fight this epidemic disease and save livelihoods in Africa. These transgenic lines are under evaluation for disease resistance and agronomic performance in field conditions Dr. K.V. Ravishankar entitled Banana: Genomics and Transgenic Approaches for Crop Improvement , is a unique blend of information on banana genomics and transgenic approach for crop improvement. The over-view of progress in this area of research has been put together by leading experts who have organized the book into two parts

development of transgenic banana (musa spp) with resistance to bacterial wilt: a risk assessment appraisal report for sub-saharan africa prepared for african agricultural technology foundation (aatf) by dr. n.o. amugune 1, prof. j.i. kinyamario and dr. j.w. kimenju2 1school of biological sciences, university of nairob Enset (Ensete ventricosum), also known as Ethiopian banana, is a food security crop for more than 20 million people in Ethiopia. As conventional breeding of enset is very challenging, genetic engineering is an alternative option to introduce important traits such as enhanced disease resistance and nutritional value. Genetic transformation and subsequent regeneration of transgenic enset has. The banana tree forms rhizomes that form into a little tree known as a pup that can be removed and planted elsewhere. Which means, they're all sterile, and each new banana plant has to be manually planted from a cutting of existing banana roots. This genetic homogeneity is of course quite risky. If a parasite or disease becomes particularly.

(PDF) Production of Transgenic Banana (Musa species

  1. The researchers pursuing transgenic bananas are currently seeking a new banana that will tuck neatly into Cavendish's slot in this system, without disturbing, or questioning, any of these other.
  2. Bananas and genetic engineering: Past, present and future. The Pahang banana -- good for research but not so good for eating because its fruits are full of seeds. Scientists have sequenced its.
  3. Transgenic banana are produced with the capacity to produce Hepatitis B vaccination and hence by eating this transgenic banana one can get immunity against this dangerous virus known as Hepatitis B. Transgenic Plant and Alzheimer's disease: 1. Alzheimer's disease is a neurodegenerative disease and it causes dementia in patients
  4. Transgenic Banana Project at UHM This work is a result of contributions Dr. John Hu made by many individuals: Dr. Eden Perez Dr. Kheng Cheah Dr. Said Khalil Dr. Diane Sether Mr. Doug Gaskill Mr. Jason Lutz Dr. Wayne Bort
  5. Here we report the identification of transgenic Cavendish with resistance to TR4. In our 3-year field trial, two lines of transgenic Cavendish, one transformed with RGA2, a gene isolated from a TR4-resistant diploid banana, and the other with a nematode-derived gene, Ced9, remain disease free. Transgene expression in the RGA2 lines is strongly.

Genetically modified bananas: To mitigate food security

  1. The transgenic banana plantlets were inoculated with 2x10 6 spores mL-1 conidial suspension of Fusarium oxysporum f. sp. cubense (Race 1) to evaluate the degree of tolerance and to investigate the effectiveness of the bioassay system as a potential tool for early screening. Assay of protein extract from the transgenic plantlets showed an.
  2. Tea and banana plants have been genetically modified by bacteria. ABOUT one in 20 flowering plants are naturally transgenic, carrying bacterial DNA within their genomes. The added genes can make.
  3. Banana (Musa spp.) is a staple food for more than 400 million people. [...] Key Result In our 3-year field trial, two lines of transgenic Cavendish, one transformed with RGA2, a gene isolated from a TR4-resistant diploid banana, and the other with a nematode-derived gene, Ced9, remain disease free. Transgene expression in the RGA2 lines is strongly correlated with resistance
  4. Transgenic bananas appear to successfully resist a bacterial wilting disease and pest species that are devastating the critical food crop in tropical regions across the planet, according to a newly published scientific paper.. The research has tremendous implications for Africa, which produces about a third of the 145 million tons of banana grown globally each year and 72 percent of the plantains
  5. In studying the effects of different salt concentrations on the produced transgenic banana plants, results showed lower decrease in the percentage of survived plants, pseudostem diameter and leaf area. with an increase of salt concentrations in case of transgenic plants compared with the controls. Record Number : 20053149457
  6. Previously, transgenic resistance to Foc race 1 in banana has been reported from glasshouse testing using either anti-apoptosis genes 8,15 or RNA interference targeting essential Foc genes 16. This is the first report of Fusarium wilt resistance in transgenic bananas in the field
  7. Figure 1: Regeneration of transgenic banana using tissue culture method. Somatic embryos are embryos that originate in tissue culture in response to plant hormones added to the growth medium. Source: National Agricultural Biotechnology Centre, Uganda. Content. Biotechnology

Genetically modified bananas with longer shelf life

from transgenic banana cv. 'Grande Naine' (Musa AAA). To achieve this, the embryo specific REG-2 promoter was isolated from rice and its expression pattern in banana cell clumps, somatic embryos and regenerated plantlets was characterized by using a pREG2::uidA fusion construct. Subsequently, the REG-2 promoter was place Banana Xanthomonas Wilt (BXW), or banana bacterial wilt (BBW) or enset wilt is a bacterial disease caused by Xanthomonas campestris pv. musacearum. After being originally identified on a close relative of banana, Ensete ventricosum, in Ethiopia in the 1960s, BXW emanated in Uganda in 2001 affecting all types of banana cultivars.Since then BXW has been diagnosed in Central and East Africa. Banana is one of the major crops that promote food security and household incomes. Most of the banana and its bi-products are consumed domestically. It, therefore, has high potential of creating wealth for farmers in many parts of the country. NAADS facilitates farmers to access high quality production inputs such as transgenic plants, fertilizers and herbicides Based on success with transgenic banana, we are trying to transfer transgenic technology from banana to enset in partnership with the Ethiopian Institute of Agricultural Research. Enset, closely related to banana, is a staple food source for over 15 million people in Ethiopia

transgenic plants were multiplied under in vitro using floral apex cultures. Attempts were also made to enhance the expression of HBsAg in the leaves of transgenic banana plants by wounding and/or treatment with plant growth regulators. This is the first report on the expression of HBsAg in transgenic banana fruits. G. B. Sunil In addition, banana cells transformed with the nucleic acid construct or construct system, banana plants generated therefrom and methods of producing same. WO2005047515A1 - Transgenic disease resistant banana - Google Patents Transgenic disease resistant banana Download PDF Info Publication number WO2005047515A1. WO2005047515A1 PCT. Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is one of the most threatening fungal diseases affecting banana plantations across the globe. It was first discovered in Australia in 1874 and has now spread to numerous different regions in the world hinting at the persistency of the pathogen. Various management strategies have been devised aiming mainly on improving the plant.

GM banana shows promise against deadly fungus strain

Transgenic lines expressing the Hrap gene under the regulation of the constitutive CaMV35S promoter were generated using embryogenic cell suspensions of two banana cultivars: 'Sukali Ndiizi' and 'Mpologoma' Why Transgenic Banana?• East Africa is the largest banana producing and consuming region in Africa.• BXW is causing an annual loss of over US$ 200 million in Uganda.• BXW attacks all banana varieties resulting in absolute crop loss.• Farmers prefer resistant varieties. 2 increased the tolerance of the resulting transgenic plants to drought stress [28]. In bananas, transgenic AQP genes can also improve drought resistance. Banana plants overexpressing MusaPIP1;2 showed significantly enhanced drought resistance compared to control plants [29]. In a study by Sreedharan, transgenic banana plant

Long Answer 1) You seek to create a transgenic banana that produces a protein used in the vaccine against polio. You need to attach the polio protein to a banana-specific promoter that is present on the plasmid pBanana so that the polio antigen will be made in bananas Transgenic banana plants expressing the antifungal chitinase RCG3 gene are being developed for efficacy against black Sigatoka. State‐of‐the-­art RNA interference (RNAi) technology is being used in transgenic bananas to selectively stop the pathogenic fungi from infecting banana plants with two diseases (black Sigatoka and fusarium wilt. Transgenic banana plants expressing high level of full-length PhDef1 and PhDef2 were phenotypically normal and no stunting was observed. In conclusion, our results suggest that high-level constitutive expression of floral defensins having distinctive prodomains is an efficient strategy for development of fungal resistance in economically.

Bananas and genetic engineering: Past, present and future

Transgenic Banana (Musa acuminata) The banana, a fourth most important food crop of the developing countries, has been predominantly targeted for beta-carotene. This has been achieved by developing transgenic banana (Super Banana) by expressing PSY gene ( PSY2a ) of Asupina banana, which is naturally high in beta-carotene ( 185 ) Determination of Copy number and genomic integrity of T-DNA in transgenic banana plants. (A) Southern analysis to determine the transgene copy number in transgenic banana plants expressing PhDef1 (D1 6, D1 12, D1 16, D1 20) and PhDef2 (D2 3, D2 4, D2 9, D2 11). UC denotes an untransformed control plant

Banana Bunchy Top Virus - Agdia EMEA

Also, transgenic tobacco with rice tlp showed enhanced resistance against Alternaria alternata. In addition, Mahdavi et al. developed transgenic banana with Oryza sativum thaumatin-like protein gene and demonstrated increased disease resistance to Fusarium wilt caused by the fungal pathogen Fusarium oxysporum sp. cubense (race 4). Therefore. Edu, F (2021). Optimization of in vitro regeneration and genetic transformation protocol for selected banana and plantain (musa spp.) Cultivars and generation of transgenic banana resistant to bacterial xan Transgenic approaches are potential tools for direct transfer of these genes into popular cultivars, which are generally not amenable for conventional breeding techniques, in specific with crops such as bananas which are sterile, triploid and heterozygous thereby making it difficult to reconstruct the recurrent genotypes in banana Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the.

Process of Developing Genetically Modified (GM) Crops

Transgenic banana plants expressing a Stellaria media

With an annual production of over 100 million metric tons bananas (including plantains) are the most important fruit crop on Earth and cultivated in close to 140, chiefly less developed countries in tropical and subtropical regions (FAOSTAT, 2005). Egypt produced 880 thousand tons (0.9% of global production) in 2005 on a surface of 28.000 hectares, which corresponds to an excellent 31 t/ha. Transgenic banana could feed millions A safe transgenic banana could prevent nematodes (worms) destroying around 6 million tonnes of bananas a year. This is enough to feed the 60 million people in Uganda, Rwanda, Ghana, Nigeria and Cameroon for whom banana is a staple food. Because bananas are sterile, it's very hard to breed resistance to. transgenic banana leaves accumulated more Fe compared to the wild type indicating that constitutive expression using 35S and Ubi promoters was more effective in the vegetative parts of banana plants compared to the fruit pulp. OsNAS1 enhanced Fe and Zn accumulation in both fruit pulp and leaf tissue although reduced Zn concentration was.

Transgenic Papaya Genome Draft Yields Many Fruits | PapayaEdible vaccinePh02 edible vaccinesBanana aphid (Pentalonia nigronervosa)

Published online: 19 November 2011. Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt diseas The Bill and Melinda Gates foundation is also supporting ongoing transgenic research. However, despite strong scientific evidence that GM foods are safe, the banana is unlikely to be on a. When a transgenic banana (or other food product) containing the HBV surface antigen is ingested, the HBV viral antigen will be encountered by microfold cells (M-cells) within the lumen of the small intestine. M-cells will recognize the antigen as foreign and possibly pathogenic, endocytosizing the protein and presenting it to the dendritic.